3D visualization of dislocation arrangement using scanning electron microscope serial sectioning method
نویسندگان
چکیده
منابع مشابه
Soft Part 3D visualization by serial sectioning and computer reconstruction
Recent increases in performance of personal computer hardand software enabled a variety of 3D graphical applications, such as surface visualizations of biological specimens. This indirectly recalled an old morphological method back to life, the investigation of soft part anatomy by light microscopical serial section analysis. A practical guide covering all procession steps beginning with anesth...
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it is only thirteen years since the scanning electron microscope has been available commercially. yet, even in this short period of time, this instrument has been a powerful tool in the investigation of topography, electrical and magnetic properties, crystal structure, cathodoluminescent characteristics etc. of solid specimens. today, this type of microscope has opened its place alongside the c...
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The use of spherical aberration correctors in the scanning transmission electron microscope (STEM) has the effect of reducing the depth of field of the microscope, making three-dimensional imaging of a specimen possible by optical sectioning. Depth resolution can be improved further by placing aberration correctors and lenses pre and post specimen to achieve an imaging mode known as scanning co...
متن کاملUniform serial sectioning for transmission electron microscopy.
Kristen M. Harris,1,2,3 Elizabeth Perry,2 Jennifer Bourne,1,2 Marcia Feinberg,3 Linnaea Ostroff,3 and Jamie Hurlburt2,3 1Section of Neurobiology and Center for Learning and Memory, University of Texas at Austin, Austin, Texas 78712, 2Department of Neurology, Synapses, and Cognitive Neuroscience Center, Medical College of Georgia, Augusta, Georgia 30912, 3Program in Neuroscience and Biology Depa...
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The processes of life take place in multiple dimensions, but imaging these processes in even three dimensions is challenging. Here, we describe a workflow for 3D correlative light and electron microscopy (CLEM) of cell monolayers using fluorescence microscopy to identify and follow biological events, combined with serial blockface scanning electron microscopy to analyse the underlying ultrastru...
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ژورنال
عنوان ژورنال: Scripta Materialia
سال: 2015
ISSN: 1359-6462
DOI: 10.1016/j.scriptamat.2015.02.001